Vitreous humour as a potential DNA source for postmortem human identification.

نویسندگان

  • Ireneusz Stołyszewski
  • Anna Niemcunowicz-Janica
  • Witold Pepiński
  • Magdalena Spólnicka
  • Renata Zbiec
  • Jerzy Janica
چکیده

PURPOSE The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification. MATERIAL AND METHODS Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles. RESULTS Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach. CONCLUSION Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Free amino acid content of the vitreous humour in cot deaths.

The concentration of 27 amino acids in the vitreous humour was analysed in 120 cot deaths. Baseline data for age and postmortem interval are presented and the concentrations compared with the normal reference range for plasma. The results do not indicate that a specific aminoacidopathy is a factor in cot deaths.

متن کامل

Interpretation of Ethanol Analysis in Postmortem Specimens

Since alcohol is a general central nervous system depressant, it affects the motor and cognitive performance. Alcohol abuse is a major cause of medical and social problems. Additionally, to determine cause of death in legal medicine centers, alcohol analysis is the most frequently performed test in forensic toxicology laboratories. Accurate interpretation of ethanol concentration at the time...

متن کامل

Ancient DNA from Human and Animal

Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...

متن کامل

Study of Gamma-Hydroxybutyric Acid (GHB) Concentrations in Postmortem Blood and Urine

The GHB concentration in majority of the blood samples (95%) was ≤ 50 mg/L, while in 81% it ranged from 10-50 mg/L. In 95% of the urine samples, the GHB concentration ranged from 10-20 mg/L while 82% of the samples had a concentration of < 10 mg/L. In cases where GHB intoxication was identified, GHB concentrations ranged from 264 to >500 mg/L. The proposed published GHB concentration of 50 mg/L...

متن کامل

MicroRNA signatures in vitreous humour and plasma of patients with exudative AMD

Age-related macular degeneration (AMD) is a leading cause of blindness worldwide affecting individuals over the age of 50. The neovascular form (NV AMD) is characterized by choroidal neovascularization (CNV) and responsible for the majority of central vision impairment. Using non-biased microRNA arrays and individual TaqMan qPCRs, we profiled miRNAs in the vitreous humour and plasma of patients...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Folia histochemica et cytobiologica

دوره 45 2  شماره 

صفحات  -

تاریخ انتشار 2007